SCIENTIFIC PAPERS
Asterisk * stands for corresponding author, bold italic for PhD & Master students under my supervision.
Submitted
- Valentine Seveau de Noray, Paulin Nègre, Martine Biarnes, Marie-Pierre Valignat, Olivier Theodoly*. On the passive or active nature of directed motility.
- Pierre Bohec, Florian Dupuy, Victoria Tishkova, Valentine Seveau de Noray, Marie-Pierre Valignat, Olivier Theodoly*. Microvalve-based devices for flow-free gradient generators.
- Marie-Pierre Valignat*, Martine Biarnes, Dominique Touchard, Audrey Rico, Patrick Chames, Olivier Theodoly*, Philippe Robert*. Bivalency of natalizumab promotes inhibition of dynamic VLA-4 adhesion beyond affinity gain.
- Lucas Curci, Valentine Seveau de Noray, Olivier Theodoly, Marie-Pierre Valignat, Florence Hubert* and Julien Olivier*. In silico phase-field modeling of cell directed migration towards environments of lower or higher adhesion.
Published
(52) Garcia-Seyda, N.; Song, S.; Seveau De Noray, V.; David-Broglio, L.; Matti, C.; Artinger, M.; Dupuy, F.; Biarnes-Pelicot, M.; Valignat, M.-P.; Legler, D. F.; Bajénoff, M.; Theodoly, O.* Naïve T Lymphocytes Chemotax Long Distance to CCL21 but Not to a Source of Bioactive S1P. iScience 2023, 107695. https://doi.org/10.1016/j.isci.2023.107695
(51) Seveau de Noray, V.; Manca, F.; Mainil, I.; Remson, A.; Biarnes-Pelicot, M.; Gabriele, S.; Valignat, M.-P.; Theodoly, O.* Keratocytes Migrate against Flow with a Roly-Poly-like Mechanism. Proc. Natl. Acad. Sci. 2022, 119 (48), e2210379119. https://doi.org/10.1073/pnas.2210379119
Commentary in the News of the CNRS
(50) Mao, Q.; Acharya, A.; Rodríguez-delaRosa, A.; Marchiano, F.; Dehapiot, B.; Al Tanoury, Z.; Rao, J.; Díaz-Cuadros, M.; Mansur, A.; Wagner, E.; Chardes, C.; Gupta, V.; Lenne, P.-F.; Habermann, B. H.; Theodoly, O.; Pourquié, O.; Schnorrer, F. Tension-Driven Multi-Scale Self-Organisation in Human IPSC-Derived Muscle Fibers. eLife 2022, 11, e76649. https://doi.org/10.7554/eLife.76649
(49) Aoun, L.; Nègre, P.; Gonsales, C.; Seveau de Noray, V.; Brustlein, S.; Biarnes-Pelicot, M.; Valignat, M.-P.; Theodoly, O.* Leukocyte Transmigration and Longitudinal Forward-Thrusting Force in a Microfluidic Transwell Device. Biophys. J. 2021, S0006349521002927. https://doi.org/10.1016/j.bpj.2021.03.037
(48) Robert, P.; Biarnes-Pelicot, M.; Garcia-Seyda, N.; Hatoum, P.; Touchard, D.; Brustlein, S.; Nicolas, P.; Malissen, B.; Valignat, M.-P.; Theodoly, O.* Functional Mapping of Adhesiveness on Live Cells Reveals How Guidance Phenotypes Can Emerge From Complex Spatiotemporal Integrin Regulation. Front. Bioeng. Biotechnol. 2021, 9, 625366. https://doi.org/10.3389/fbioe.2021.625366.
(47) Luo, X.; Noray, V. S. de; Aoun, L.; Biarnes-Pelicot, M.; Strale, P.-O.; Studer, V.; Valignat, M.-P.; Theodoly, O.* Lymphocytes Perform Reverse Adhesive Haptotaxis Mediated by LFA-1 Integrins. J. Cell Sci. 2020, 133 (16). https://doi.org/10.1242/jcs.242883
(46) Versaevel, M.; Alaimo, L.; Seveau, V.; Luciano, M.; Mohammed, D.; Bruyere, C.; Vercruysse, E.; Theodoly, O.; Gabriele, S. Collective Migration during a Gap Closure in a Two-Dimensional Haptotactic Model. Sci. Rep. 2021, 11 (1), 5811. https://doi.org/10.1038/s41598-021-84998-w.
(45) Garcia-Seyda, N.; Seveau, V.; Manca, F.; Biarnes-Pelicot, M.; Valignat, M.-P.; Bajenoff, M.; Theodoly, O.* Human Neutrophils Swim and Phagocytise Bacteria. Biol. Cell 2021, 113 (1), 28–38. https://doi.org/10.1111/boc.202000084
(44) Sadoun, A.; Biarnes-Pelicot, M.; Ghesquiere-Dierickx, L.; Wu, A.; Theodoly, O.; Limozin, L.; Hamon, Y.; Puech, P.-H. Controlling T Cells Spreading, Mechanics and Activation by Micropatterning. Sci. Rep. 2021, 11 (1), 6783. https://doi.org/10.1038/s41598-021-86133-1
(43) Mohammed, D.; Charras, G.; Vercruysse, E.; Versaevel, M.; Lantoine, J.; Alaimo, L.; Bruyère, C.; Luciano, M.; Glinel, K.; Delhaye, G.; Théodoly, O.; Gabriele, S. Substrate Area Confinement Is a Key Determinant of Cell Velocity in Collective Migration. Nat. Phys. 2019, 15 (8), 858–866. https://doi.org/10.1038/s41567-019-0543-3
(42) He, L.; Valignat, M.-P.; Zhang, L.; Gelard, L.; Zhang, F.; Le Guen, V.; Audebert, S.; Camoin, L.; Fossum, E.; Bogen, B.; Wang, H.; Henri, S.; Roncagalli, R.; Theodoly, O.; Liang, Y.; Malissen, M.; Malissen, B. ARHGAP45 Controls Naive T- and B- Cell Entry into Lymph Nodes and T-Cell Progenitor Thymus Seeding. Embo Rep. 2021, 22 (4), e52196. https://doi.org/10.15252/embr.202052196
(41) Rasedujjaman, M.; Affannoukou, K.; Garcia-Seyda, N.; Robert, P.; Giovannini, H.; Chaumet, P. C.; Theodoly, O.; Valignat, M.-P.; Belkebir, K.; Sentenac, A.; Maire, G. Three-Dimensional Imaging with Reflection Synthetic Confocal Microscopy. Opt. Lett. 2020, 45 (13), 3721–3724. https://doi.org/10.1364/OL.397364
(40) Aoun, L.; Farutin, A.; Garcia-Seyda, N.; Nègre, P.; Rizvi, M. S.; Tlili, S.; Song, S.; Luo, X.; Biarnes-Pelicot, M.; Galland, R.; Sibarita, J.-B.; Michelot, A.; Hivroz, C.; Rafai, S.; Valignat, M.-P.; Misbah, C.; Theodoly, O.* Amoeboid Swimming Is Propelled by Molecular Paddling in Lymphocytes. Biophys. J. 2020, 119 (6), 1157–1177. https://doi.org/10.1016/j.bpj.2020.07.033
Highlighted in:Biophysical Journal; Science; Eurekalert; Science&Vie
Quoted and commented in Nature reviews:” This fascinating recent work demonstrates a new mechanism of cell swimming using ‘molecular paddling’ of cell surface proteins coupled to flow of the underlying cell cortex.”
(39) Garcia-Seyda, N.; Aoun, L.; Tishkova, V.; Seveau, V.; Biarnes-Pelicot, M.; Bajénoff, M.; Valignat, M.-P.; Theodoly, O.* Microfluidic Device to Study Flow-Free Chemotaxis of Swimming Cells. Lab. Chip 2020, 20 (9), 1639–1647. https://doi.org/10.1039/D0LC00045K
(38) Hornung, A.; Sbarrato, T.; Garcia-Seyda, N.; Aoun, L.; Luo, X.; Biarnes-Pelicot, M.; Theodoly, O.*; Valignat, M.-P.* A Bistable Mechanism Mediated by Integrins Controls Mechanotaxis of Leukocytes. Biophys. J. 2020, 118 (3), 565–577. https://doi.org/10.1016/j.bpj.2019.12.013
(37) Reichert, B.; Huerre, A.; Theodoly, O.; Valignat, M.-P.; Cantat, I.; Jullien, M.-C. Topography of the Lubrication Film under a Pancake Droplet Travelling in a Hele-Shaw Cell. J. Fluid Mech. 2018, 850, 708–732. https://doi.org/10.1017/jfm.2018.457.
(36) Treguier, J.; Bugnicourt, L.; Gay, G.; Diallo, M.; Islam, S. T.; Toro, A.; David, L.; Theodoly, O.; Sudre, G.; Mignot, T. Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing. Mbio 2019, 10 (4), e01375-19. https://doi.org/10.1128/mBio.01375-19.
(35) Theodoly, O.*;Garcia-Seyda, N.; Bedu, F.; Luo, X.; Gabriele, S.; Mignot, T.; Giermanska, J.; Chapel, J.-P.; Metivier, M.; Valignat, M.-P. Live Nanoscopic to Mesoscopic Topography Reconstruction with an Optical Microscope for Chemical and Biological Samples. Plos One 2018, 13 (12), e0207881. https://doi.org/10.1371/journal.pone.0207881
(34) Huerre, A.; Valignat, M.-P.; Maggs, A. C.; Theodoly, O.*; Jullien, M.-C.* Laplace Pressure Based Disjoining Pressure Isotherm in Non Symmetric Conditions. Appl. Phys. Lett. 2017, 111 (22), 221601. https://doi.org/10.1063/1.4997857
(33) Faure, L. M.; Fiche, J.-B.; Espinosa, L.; Ducret, A.; Anantharaman, V.; Luciano, J.; Lhospice, S.; Islam, S. T.; Treguier, J.; Sotes, M.; Kuru, E.; Van Nieuwenhze, M. S.; Brun, Y. V.; Theodoly, O.; Aravind, L.; Nollmann, M.; Mignot, T. The Mechanism of Force Transmission at Bacterial Focal Adhesion Complexes. Nature 2016, 539 (7630), 530-+. https://doi.org/10.1038/nature20121.
(32) Huerre, A.; Jullien, M.-C.; Theodoly, O.*; Valignat, M.-P.* Absolute 3D Reconstruction of Thin Films Topography in Microfluidic Channels by Interference Reflection Microscopy. Lab Chip 2016, 16 (5), 911–916. https://doi.org/10.1039/C5LC01417D.
(31) Hubert, F.; Jedouaa, M.; Khames, I.; Olivier, J.; Theodoly, O.; Trescases, A. Cell Motility in Confinement: A Computational Model for the Shape of the Cell. ESAIM Proc. Surv. 2016, 55, 148–166.
(30) Preira, P.; Forel, J.-M.; Robert, P.; Nègre, P.; Biarnes-Pelicot, M.; Xeridat, F.; Bongrand, P.; Papazian, L.; Theodoly, O.* The Leukocyte-Stiffening Property of Plasma in Early Acute Respiratory Distress Syndrome (ARDS) Revealed by a Microfluidic Single-Cell Study: The Role of Cytokines and Protection with Antibodies. Crit. Care 2016, 20 (1), 1. https://doi.org/10.1186/s13054-015-1157-5.
(29) Huerre, A.; Theodoly, O.; Leshansky, A. M.; Valignat, M.-P.; Cantat, I.; Jullien, M.-C. Droplets in Microchannels: Dynamical Properties of the Lubrication Film. Phys. Rev. Lett. 2015, 115 (6). https://doi.org/10.1103/PhysRevLett.115.064501.
(28) Cadra, S.; Gucciardi, A.; Valignat, M.-P.; Theodoly, O.; Vacaflores, A.; Houtman, J. C. D.; Lellouch, A. C. ROZA-XL, an Improved FRET Based Biosensor with an Increased Dynamic Range for Visualizing Zeta Associated Protein 70 KD (ZAP-70) Tyrosine Kinase Activity in Live T Cells. Biochem. Biophys. Res. Commun. 2015, 459 (3), 405–410. https://doi.org/10.1016/j.bbrc.2015.02.117.
(27) Valignat, M.-P.; Nègre, P.; Cadra, S.; Lellouch, A. C.; Gallet, F.; Hénon, S.; Theodoly, O.* Lymphocytes Can Self-Steer Passively with Wind Vane Uropods. Nat. Commun. 2014, 5, 5213. https://doi.org/10.1038/ncomms6213.
(26) Preira, P.; Valignat, M.-P.; Bico, J.; Théodoly, O.* Single Cell Rheometry with a Microfluidic Constriction: Quantitative Control of Friction and Fluid Leaks between Cell and Channel Walls. Biomicrofluidics 2013, 7 (2), 024111. https://doi.org/10.1063/1.4802272.
(25) Valignat, M.-P.; Theodoly, O.; Gucciardi, A.; Hogg, N.; Lellouch, A. C. T Lymphocytes Orient against the Direction of Fluid Flow during LFA-1-Mediated Migration. Biophys. J. 2013, 104 (2), 322–331. https://doi.org/10.1016/j.bpj.2012.12.007.
(24) Preira, P.; Grandné, V.; Forel, J.-M.; Gabriele, S.; Camara, M.; Theodoly, O.* Passive Circulating Cell Sorting by Deformability Using a Microfluidic Gradual Filter. Lab Chip 2013, 13 (1), 161–170. https://doi.org/10.1039/C2LC40847C.
(23) Ducret, A.; Theodoly, O.; Mignot, T. Single Cell Microfluidic Studies of Bacterial Motility. Methods Mol. Biol. Clifton NJ 2013, 966, 97–107. https://doi.org/10.1007/978-1-62703-245-2_6.
(22) Qi, L.; Fresnais, J.; Muller, P.; Theodoly, O.; Berret, J.-F.; Chapel, J.-P. Interfacial Activity of Phosphonated-PEG Functionalized Cerium Oxide Nanoparticles. Langmuir 2012, 28 (31), 11448–11456. https://doi.org/10.1021/la302173g.
(21) Ducret, A.; Valignat, M.-P.; Mouhamar, F.; Mignot, T.*; Theodoly, O.* Wet-Surface-Enhanced Ellipsometric Contrast Microscopy Identifies Slime as a Major Adhesion Factor during Bacterial Surface Motility. Proc. Natl. Acad. Sci. U. S. A. 2012, 109 (25), 10036–10041. https://doi.org/10.1073/pnas.1120979109.
(20) Preira, P.; Leoni, T.; Valignat, M.-P.; Lellouch, A.; Robert, P.; Forel, J.-M.; Papazian, L.; Dumenil, G.; Bongrand, P.; Theodoly, O.* Microfluidic Tools to Investigate Pathologies in the Blood Microcirculation. Int. J. Nanotechnol. 2012, 9 (3–7), 529–547. https://doi.org/10.1504/IJNT.2012.045340.
(19) Jacquin, M.; Muller, P.; Cottet, H.; Théodoly, O.* Self-Assembly of Charged Amphiphilic Diblock Copolymers with Insoluble Blocks of Decreasing Hydrophobicity: From Kinetically Frozen Colloids to Macrosurfactants. Langmuir 2010, 26 (24), 18681–18693. https://doi.org/10.1021/la103391p.
(18) Gabriele, S.; Versaevel, M.; Preira, P.; Théodoly, O.* A Simple Microfluidic Method to Select, Isolate, and Manipulate Single-Cells in Mechanical and Biochemical Assays. Lab. Chip 2010, 10 (11), 1459–1467. https://doi.org/10.1039/C002257H.
(17) Theodoly, O.*; Checco, A.; Muller, P.* Charged Diblock Copolymers at Interfaces: Micelle Dissociation upon Compression. EPL Europhys. Lett. 2010, 90 (2), 28004. https://doi.org/10.1209/0295-5075/90/28004.
(16) Theodoly, O.; Huang, Z.-H.; Valignat, M.-P. New Modeling of Reflection Interference Contrast Microscopy Including Polarization and Numerical Aperture Effects: Application to Nanometric Distance Measurements and Object Profile Reconstruction. Langmuir ACS J. Surf. Colloids 2010, 26 (3), 1940–1948. https://doi.org/10.1021/la902504y.
(15) Gabriele, S.; Benoliel, A.-M.; Bongrand, P.; Theodoly, O.* Microfluidic Investigation Reveals Distinct Roles for Actin Cytoskeleton and Myosin II Activity in Capillary Leukocyte Trafficking. Biophys. J. 2009, 96 (10), 4308–4318. https://doi.org/10.1016/j.bpj.2009.02.037.
(14) Theodoly, O.*; Jacquin, M.; Muller, P.; Chhun, S. Adsorption Kinetics of Amphiphilic Diblock Copolymers: From Kinetically Frozen Colloids to Macrosurfactants. LANGMUIR 2009, 25 (2), 781–793. https://doi.org/10.1021/la8030254.
(13) Muller, P.; Sudre, G.; Theodoly, O.* Wetting Transition on Hydrophobic Surfaces Covered by Polyelectrolyte Brushes. Langmuir 2008, 24 (17), 9541–9550. https://doi.org/10.1021/la801406x.
(12) Theodoly, O.; Gabriele, S.; Valignat, M.-P. Influence of Surface Reflective Properties on Differential Interference Contrast Microscopy. Opt. Express 2008, 16 (7), 4547–4558.
(11) Jacquin, M.; Muller, P.; Talingting-Pabalan, R.; Cottet, H.; Berret, J.; Futterer, T.; Theodoly, O.* Chemical Analysis and Aqueous Solution Properties of Charged Amphiphilic Block Copolymers PBA-b-PAA Synthesized by MADIX((c)). J. COLLOID INTERFACE Sci. 2007, 316 (2), 897–911. https://doi.org/10.1016/j.jcis.2007.08.025.
(10) Jacquin, M.; Muller, P.; Cottet, H.; Crooks, R.; Theodoly, O.* Controlling the Melting of Kinetically Frozen Poly(Butyl Acrylate-b-Acrylic Acid) Micelles via Addition of Surfactant. LANGMUIR 2007, 23 (20), 9939–9948. https://doi.org/10.1021/1a700370f.
(9) Jacquin, M.; Muller, P.; Lizarraga, G.; Bauer, C.; Cottet, H.; Theodoly, O.* Characterization of Amphiphilic Diblock Copolymers Synthesized by MADIX Polymerization Process. MACROMOLECULES 2007, 40 (8), 2672–2682. https://doi.org/10.1021/ma062600+.
(8) Theodoly, O.; Cascao-Pereira, L.; Bergeron, V.; Radke, C. A Combined Streaming-Potential Optical Reflectometer for Studying Adsorption at the Water/Solid Surface. LANGMUIR 2005, 21 (22), 10127–10139. https://doi.org/10.1021/la050685m.
(7) Baigl, D.; Sferrazza, M.; Guedeau-Boudeville, M.; Ober, R.; Rieutord, F.; Theodoly, O.; Williams, C. In Situ High-Energy x-Ray Reflectivity Studies of Hydrophobic Polyelectrolytes at the Solid/Liquid Interface. J. Phys.-Condens. MATTER 2005, 17 (41), 6329–6336. https://doi.org/10.1088/0953-8984/17/41/004.
(6) Valignat, M.; Theodoly, O.; Crocker, J.; Russel, W.; Chaikin, P. Reversible Self-Assembly and Directed Assembly of DNA-Linked Micrometer-Sized Colloids. Proc. Natl. Acad. Sci. U. S. OF 2005, 102 (12), 4225–4229. https://doi.org/10.1073/pnas.0500507102.
(5) Pereira, L.; Theodoly, O.; Blanch, H.; Radke, C. Dilatational Rheology of BSA Conformers at the Air/Water Interface. LANGMUIR 2003, 19 (6), 2349–2356. https://doi.org/10.1021/la020720e.
(4) Svitova, T.; Theodoly, O.; Christiano, S.; Hill, R.; Radke, C. Wetting Behavior of Silicone Oils on Solid Substrates Immersed in Aqueous Electrolyte Solutions. LANGMUIR 2002, 18 (18), 6821–6829. https://doi.org/10.1021/la02006x.
(3) Theodoly, O.; Tan, J. S.; Ober, R.; Williams, C. E.; Bergeron, V. Oscillatory Forces from Polyelectrolyte Solutions Confined in Thin Liquid Films. Langmuir 2001, 17 (16), 4910–4918.
(2) Theodoly, O.; Ober, R.; Williams, C. Adsorption of Hydrophobic Polyelectrolytes at the Air/Water Interface: Conformational Effect and History Dependence. Eur. Phys. J. E 2001, 5 (1), 51–58.
(1) Cottet, H.; Gareil, P.; Theodoly, O.; Williams, C. A Semi-Empirical Approach to the Modeling of the Electrophoretic Mobility in Free Solution: Application to Polystyrenesulfonates of Various Sulfonation Rates. ELECTROPHORESIS 2000, 21 (17), 3529–3540.
PATENTS
-2005: Block copolymer for use as an emulsifier for e.g. a pharmaceutical formulation, comprises a controlled structure comprising a hydrophilic part, and a part comprising units derived from e.g. diethyleneglycolethyletheracrylate
Patent Number: WO2005021612-A1 EP1660548-A1 CN1852930-A JP2007504311-W EP1660548-B1 DE602004016178-E US2009018270-A1 JP4473270-B2 CN1852930-B
Patent Assignee: RHODIA INC; CENT NAT RECH SCI; CNRS CENT NAT RECH SCI; et. al
Inventor(s): CROOKS R; JACQUIN M; MORVAN M; THEODOLY O
-2006: Hydrophilizing hydrophobic surface of substrate, e.g. hydrophilized fabric, preferably nonwoven or polyolefin fabric used in absorbent article, e.g. diapers by depositing polymer that is soluble in water
Patent Number: WO2006063284-A2 US2006154544-A1 EP1825043-A2 US7494944-B2 WO2006063284-A3 EP1825043-B1 EP1825043-A4
Patent Assignee: RHODIA INC; TALINGTING-PABALAN R; MORVAN M; THEODOLY O
Inventor(s): TALINGTING-PABALAN R; MORVAN M; LIZARRAGA G; THEODOLY O
-2019: Film of chitosan and a device comprising the same deposited on a substrate and uses thereof
Publication number: 20210215694
Filed: May 29, 2019
Publication date: July 15, 2021
Inventors: TÂM MIGNOT, OLIVIER THEODOLY, GUILLAUME SUDRE, LAURENT DAVID, LOÏC BUGNICOURT-MOREIRA, JULIE TREGUIER
-2022 : Micro-impression d’anticorps et de biomolecules pour le phenotypage et l’activation cellulaires
Demande internationale N° PCT/EP2023/063407 déposée le 17/05/2023 issue de la demande Europe N° 22305735.7 déposée le 18/05/2022
Inventeurs : O Theodoly, P Robert, G Delhaye
Aux noms de : Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université d’Aix-Marseille
Adhesion & Inflammation Lab 