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Principe of flow chamber automata and new modalities

Overlay, confocal, of patterned cells

We code, yes, we code

Lung epithelial cells of transgenic mouse expressing the rainbow transgene.

Optical microscopy image in live conditions and without fluorescent staining (taken in Wet-SEEC mode) of the slime deposited in the wake of gliding Myxococcus xanthus bacteria. The thickness of the deposition is less than 5 nm (Ducret et al., PNAS 2012)

Feeding a Toxoplasma gondii oocyte to a macrophage

Jurkat cells as seen using a DualView setup, transfected with FRET based ROZA sensor.

SEEC microscopy : visualization & quantification of nm films

AFM imaging of patterned cells

Stimulating calcium probe loaded immune cells (Cazaux et al Ultramicroscopy 2016)

Interference microscopy of T cells on supported lipid bilayers

Passage of a THP-1 leukocyte through a 4 μm wide constriction (Preira et al., Int J Nanotechnol, 2012; Gabriele et al., Lab on a chip 10:1459, 2010; Gabriele et al. Biophys J 96:4308, 2009)

Live imaging of actin and membrane on T cells

Biomembrane force probe configurations

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Alveole (fluorescent) protein printing

Confocal images of patterned cells

Single molecule measurements of TCR/pMHC interactions on living T cells (Puech et al, PLoS Ones 2011)

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Prototype of diagnostic microfluidic chip to sort cells by size/deformability (Preira et al. Lab Chip 2013)

Flow chamber simulations

Fluorescence imaging on T cells on supported bilayers