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SEEC microscopy : visualization & quantification of nm films

Interference microscopy of T cells on supported lipid bilayers

Passage of a THP-1 leukocyte through a 4 μm wide constriction (Preira et al., Int J Nanotechnol, 2012; Gabriele et al., Lab on a chip 10:1459, 2010; Gabriele et al. Biophys J 96:4308, 2009)

Fluorescence imaging on T cells on supported bilayers

Optical microscopy image in live conditions and without fluorescent staining (taken in Wet-SEEC mode) of the slime deposited in the wake of gliding Myxococcus xanthus bacteria. The thickness of the deposition is less than 5 nm (Ducret et al., PNAS 2012)

Biomembrane force probe configurations

Live imaging of actin and membrane on T cells

Alveole (fluorescent) protein printing

Principe of flow chamber automata and new modalities

Jurkat cells as seen using a DualView setup, transfected with FRET based ROZA sensor.

Overlay, confocal, of patterned cells

Lung epithelial cells of transgenic mouse expressing the rainbow transgene.

Feeding a Toxoplasma gondii oocyte to a macrophage

Confocal images of patterned cells

Single molecule measurements of TCR/pMHC interactions on living T cells (Puech et al, PLoS Ones 2011)

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Prototype of diagnostic microfluidic chip to sort cells by size/deformability (Preira et al. Lab Chip 2013)

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AFM imaging of patterned cells

We code, yes, we code

Flow chamber simulations

Stimulating calcium probe loaded immune cells (Cazaux et al Ultramicroscopy 2016)