Gallery Alveole (fluorescent) protein printing Jurkat cells as seen using a DualView setup, transfected with FRET based ROZA sensor. Optical microscopy image in live conditions and without fluorescent staining (taken in Wet-SEEC mode) of the slime deposited in the wake of gliding Myxococcus xanthus bacteria. The thickness of the deposition is less than 5 nm (Ducret et al., PNAS 2012) Cover Overlay, confocal, of patterned cells Flow chamber simulations AFM imaging of patterned cells Live imaging of actin and membrane on T cells Prototype of diagnostic microfluidic chip to sort cells by size/deformability (Preira et al. Lab Chip 2013) Interference microscopy of T cells on supported lipid bilayers Fluorescence imaging on T cells on supported bilayers Passage of a THP-1 leukocyte through a 4 μm wide constriction (Preira et al., Int J Nanotechnol, 2012; Gabriele et al., Lab on a chip 10:1459, 2010; Gabriele et al. Biophys J 96:4308, 2009) Stimulating calcium probe loaded immune cells (Cazaux et al Ultramicroscopy 2016) We code, yes, we code SEEC microscopy : visualization & quantification of nm films Cover Single molecule measurements of TCR/pMHC interactions on living T cells (Puech et al, PLoS Ones 2011) Biomembrane force probe configurations Confocal images of patterned cells Principe of flow chamber automata and new modalities Feeding a Toxoplasma gondii oocyte to a macrophage Lung epithelial cells of transgenic mouse expressing the rainbow transgene.