L. Limozin

Collaboration: P. Chames (CRCM, Marseille), G. Bellot (CBS, Montpellier), T. Fiodelisio-Coll (UNAM, Mexico)

The study of Immune and cancer cell interactions is of paramount importance for cancer immunotherapy and can be controlled by immune cell engagers. These molecules can recognize simultaneously tumour associated antigens as well as activating receptors on immune effector cells like T-lymphocytes and NK-cells. In collaboration with the Cancerology Institute of Marseille, we design new engagers based on hybrid origamis – monodomain antibodies assemblies. We have developed specific assays to study these agents at the interface of immune cells / surrogate cancer cells, or in co-cultures of immune and cancer cells. In this context, Celldetective, a dedicated software for full analysis of live microscopy of interacting cells, has been developed in the laboratory.

Funded by ANR DNAnobodies (Coordinator L. Limozin)

Tools are also developped in the frame of the FR/MEX IRP BioPhysImmuno (Coord. PH Puech)

Celldetective: an AI-enhanced image analysis tool for unraveling dynamic cell interactions, BioRxiv (2024)

Decoupling individual host response and immune cell engager cytotoxic potency. Cristina Gonzalez Gutierrez, et al. ACS Nano 2025, https://doi.org/10.1021/acsnano.4c08541