This project intends to perform quantitative analysis of some essential steps of the leukocyte recruitment cascade using in vitro tools developed at LAI based on microfluidics and protein patterning. The in vitro approach will be based on and compared with in vivo models of T cell traffic in lymph node, T cell response to infection by Bordetella pertussis, and T cell properties of patients with multiple sclerosis. This project is a LAI collaboration with Alveole company (Luc Talini, Romuald Valy), Vincent Studer (IINS, Bordeaux), Claire Hivroz (Institut Curie, Paris) Marc Bajénoff and Jean-Pierre Gorvel (CIML), as well as Philippe Robert and Audrey Ricco (AP-HM).