External seminar : Jonathan Fouchard

Title: Characterizing the mechanical properties of epithelial monolayers through compression and fracture.

By : Jonathan Fouchard, G. Charras Lab, London, UK


To date, much research has focused on how tissues build and repair themselves, as well as how their integrity is maintained. Yet, intriguingly, almost nothing is known about how living tissues crack and what the mechanical and biological conditions leading to this rupture are. Here I will present a systematic analysis of fracture tests performed on suspended epithelial monolayers. These monolayers are devoid of ECM and are a minimal model representative of many epithelia involved in early development. In our experiments, monolayers are cultured between two flexible rods which allow the monolayer to be stretched in a controlled manner. The formation of experimenter-induced or self-induced cracks is then monitored in the aim to identify the biological actors involved in tissue resistance.

Within the same assay, we also study the response of epithelia to compressive stress. We find that buckling of the tissue can spontaneously be reversed through cell shape changes controlled by the acto-myosin cytoskeleton. This lead us to the definition of a rheological model including visco-elasticity and contractility.

External seminars, < 2016


4/12/2015    Anna Labernardie (Barcelone, Spain)
27/11/2015    Giovanni Cappello (Liphys, Grenoble)
26/11/2015    Pierre Mangeol (IBDML, Marseille)
20/11/2015    Sébastien Sart (Ladhyx, Palaiseau)
19/11/2015    Jérôme Hottin (CBS, Montpellier)
16/11/2015    Andrey Zavialov (Turku, Finland)
6/11/2015    Martial Balland (Liphy, Grenoble)
16/10/2015    Cécile Bidan (Liphy, Grenoble)
29/9/2015    Demosthène Mitrossilis (Institut Curie, Paris)

12/12/2014    Guillaume Gay (DAMCB, Marseille)
5/12/2014    Adrien Ducret (Indiana Univ., USA)
24/11/2014    Clemens Franz (Karlsruhe, Germany)
7/11/2014    Sylvain Gabriele (Mons, Belgium)
12/9/2014    Karine Anselme (IS2M, Mulhouse)
13/6/2014    Guillaume Voisinne (CIML, Marseille)
6/6/2014    Daniel Jost (ENS Lyon)
14/5/2014    Lena Svensson (Lund, Sweden)
12/5/2014    Cyrille Mionnet (Gand, Belgium)

18/10/2013    Claire Hivroz (Institut Curie, Paris)
6/9/2013    Lionel Foret (LPS, Paris)
6/6/2013    Arnaud Millet (Institut Cochin, Paris)
24/5/2013    Julien Textoris (APHM, Marseille)
25/1/2013    Aurélien Dumètre (AMU, Marseille)

14/12/2012    Salvatore Valitutti (Centre Physiologie, Toulouse)
15/10/2012    François Robin (Chicago, USA)
12/10/2012    Dimitri Missirlis (Heildeberg, Germany)
12/7/2012    Zhan Hua Silber Li (Beijing, China)
15/6/2012    Abdul Barakat (Ladhyx, Palaiseau)
23/3/2012    Delphine Muriaux, Cyrille Favard (CPBS, Montpellier)

29/9/2011    Ulrich Blank (Inserm Bichat, Paris)
01/7/2011    Atef Asnacios (MSC, Paris)
10/6/2011    Catherine Even (LPS, Orsay)
13/5/2011    Patricia Veras, Washington Dos Santos  (Salvador, Brasil)
18/3/2011    Marc Bajenoff (CIML, Marseille)