A new article on three-dimensional reconstruction of the cell lamellipod without need of fluorescent labelling by using Interferential Nanoscopy in Nano Letters

Long-time expertise of LAI  (in collaboration with CINAM) in Reflection interference contrast microscopy have recently permitted a new advance to perform nanometer scale mapping of the cell lamellipod in vitro. Combining multicolor RICM with in silico reconstruction and using optical modeling, the topography of the upper and lower membrane of a lamellipod was reconstructed, as well as the refractive index mapped. These measurements were successfully compared with independent determinations using Atomic Force Microscopy or Quantitative Phase Imaging which both provide only a partial information.


3D representations of basal membrane (left: seen from under) and of apical membrane (right : seen from top).