L. Limozin and P. Robert
The laminar flow chamber is a potent tool to quantify 2D bond formation and rupture at the single molecular level in a highly parallelized assay (Robert 2011, Lo Schiavo 2012). Functionalized microbeads are convected above a surface coated with complementary ligands at low density. Videorecording and analyses of bead trajectories provide the frequency of bond formation as well as bond duration. The laminar flow is controlling the duration or molecular encounter leading to bond formation, as well as the force applied to the bond once it is formed. An original automaton, designed and built in LAI, has been used to measure molecular interactions under controlled mechanical and temperature conditions (Limozin 2016).
It was in particular applied to TCR-pMHC interactions (Robert 2012; Limozin in preparation) and antigen-antibody recognition (Robert 2011; Limozin 2016; Gonzalez 2019)
Gonzalez C, Chames P, Kerfelec B, Baty D, Robert P, Limozin L (2019). Biophys. J. 116: 1516-1526. Pubmed.
Limozin L, Bongrand P , Robert P (2016) Sci. Rep. 6:35193. Pubmed
Lo Schiavo V, P. Robert, L. Limozin, P. Bongrand (2012) PLoS ONE 7:e44070. Pubmed
Robert P , Nicolas A, Aranda-Espinoza S, Bongrand P, Limozin L (2011) Biophys. J. 100 : 2642-2651. Pubmed
Robert P, M Aleksic, O Dushek, V Cerundolo, P. Bongrand, PA van der Merwe (2012) . Biophys. J. 102:248-257. Pubmed
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